Strain Information | |
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Image | |
BRC No. | RBRC02398 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129X1-Pknox2<tm1.1Nuk> |
Former Common name | Prep2LacZKIdeltaNeo, C57BL/6 |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Atsushi Kuroiwa, Graduate School of Science, Nagoya University. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette. PGK-neo was excised by crossing with mice expressing cre recombinase. |
Strain description | Prep2(Pknox2) is a transcriptional regulator having TALE momeodomain, and is involved in transcriptinal regulation by binding to the target sequence as a heterodimer with transcription factor of Pbx having TALE homeodomain. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette. PGK-neo was excised by crossing with mice expressing cre recombinase. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pknox2Pbx/knotted 1 homeobox 29Pknox2<tm1.1Nuk>targeted mutation 1.1, Nagoya University Atsushi KuroiwaD230005H23Rik, Prep2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ASV40 polyA9poly A |
Ordering Information | |
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Donor DNA | phage P1 loxP site, E. coli LacZ, SV40 polyA additional signal sequence, mouse Pknox2 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Atsushi Kuroiwa (Nagoya University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |