Strain Data Sheet

RBRC02400

Strain Information

Image
BRC No.RBRC02400
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129-Tekt2<Gt(Betageo)1Htan>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMol. Cell. Biol., 24, 7958-7964 (2004). 15340058

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tekt2tektin 24Tekt2<Gt(Betageo)1Htan>gene trap insertion 1, Hiromitsu Tanakatektin-t

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
3'-LTRretrovirus LTR (viral long terminal repeat)43'-LTR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
5'-LTRretrovirus LTR (viral long terminal repeat)45'-LTR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor4SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor4Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)4puromouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAThe gene trap vector contained a retroviral 5'-end long terminal (LTR), splice acceptor (SA), E. coli lacZ/neomycin phosphotransferase fusion gene(BetaGeo), pA, mouse phosphoglycerate kinase (PGK)-1 gene promoter, Streptomyces alboniger puromycin N-acetyltransfetase (pac) gene, splice donor (SD) sequence, 3'-LTR
Research applicationReproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If interested in obtaining materials from the tektin-t knockout mouse line, please contact the Texas Institute for Genomic Medicine directly at www.tigm.org.
DepositorHiromitsu Tanaka (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data