Strain Data Sheet

RBRC02405

Strain Information

Image
BRC No.RBRC02405
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-4930583H14Rik<tm1Hmd>
Former Common name4930583H14Rik flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
4930583H14RikRIKEN cDNA 4930583H14 gene34930583H14Rik<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)3internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tag3myc-tag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, epitope tag(mycTAG), yeast FRT (flipase recombination target) site, Phage P1 Cre recombination target sites(loxp), E. coli neo
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data