Strain Information | |
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Image | |
BRC No. | RBRC02565 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc |
Former Common name | Nanos3-CrepA(neo)#35 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Yumiko Saga (2007). |
Strain description | These knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile. |
Colony maintenance | Sibling Mating (Hemizygote x Wild-type) |
References | Dev. Biol., 318, 133-142 (2008). 18436203 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase8cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse nanos3 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 318, 133-142 (2008).Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors. |
Depositor | Yumiko Saga (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ogonuki N, Kyogoku H, Hino T, Osawa Y, Fujiwara Y, Inoue K, Kunieda T, Mizuno S, Tateno H, Sugiyama F, Kitajima TS, Ogura A. Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes. EMBO Rep 23(7) e54992(2022) 35587095 |