Strain Data Sheet

RBRC02565

Strain Information

Image
BRC No.RBRC02565
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc
Former Common nameNanos3-CrepA(neo)#35
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga (2007).
Strain descriptionThese knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile.
Colony maintenanceSibling Mating (Hemizygote x Wild-type)
ReferencesDev. Biol., 318, 133-142 (2008). 18436203

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nanos3nanos homolog 3 (Drosophila)8Nanos3<tm2(cre)Ysa>targeted mutation 2 (cre), Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse nanos3 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 318, 133-142 (2008).Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Ogonuki N, Kyogoku H, Hino T, Osawa Y, Fujiwara Y, Inoue K, Kunieda T, Mizuno S, Tateno H, Sugiyama F, Kitajima TS, Ogura A.
Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes.
EMBO Rep 23(7) e54992(2022) 35587095