Strain Information | |
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Image | |
BRC No. | RBRC02698 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Rapgef2<tm1.1Tkat> |
Former Common name | RA-GEF-1<+/-> |
H-2 Haplotype | |
ES Cell line | E14TG2a [129P2/OlaHsd] |
Background strain | C57BL/6 (semicongenic) |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. The mice were crossed with Tg(ACTB-cre)13Miya mice. C57BL/6 semicongenic. |
Strain description | RA-GEF-1 (Rapgef2) gene knockout mice. Exon 15 was removed. Homozygous knockout mice are embryonic lethal due to an abnormal vascular development. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Biochem. Biophys. Res. Commun., 363, 106-112 (2007). 17826737 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rapgef2Rap guanine nucleotide exchange factor (GEF) 23Rapgef2<tm1.1Tkat>targeted mutation 1.1, Tohru Kataoka5830453M24Rik, CNRasGEF, mKIAA0313, nRapGEP, Pdzgef1, RA-GEF-1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, mouse Rapgef2 genomic DNA |
Research application | Cell Biology Research Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 363, 106-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Tohru Kataoka (Kobe University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |