|
RBRC No. |
RBRC02699
|
|
Type |
Targeted Mutation Congenic |
 |
|
Species |
Mus musculus |
|
Strain name |
B6.Cg-Rapgef6<tm1.1Tkat> |
|
Former Common name |
RA-GEF-2<-/-> |
|
H-2 Haplotype |
No Data |
|
Background strain |
C57BL/6JJcl |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Dr. Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. The exon 21 of Rapgef6 gene was flanked by loxP sites (Rapgef6<tm1Tkat>) and removed by cre-mediated recombination following a cross to Tg(ACTB-cre)13Miya mice. |
|
Strain description |
B6;129P2(Cg)-Rapgef6<tm1.1Tkat>. RA-GEF-2 (Rapgef6) gene knock-out mice. Homozygous mutant mice show an enlarged spleen, increased IgE and IgG levels and altered cytokine production. |
|
Colony maintenance |
Heterozygote x Wild-type [C57BL/6JJcl]
Homozygous mutant mice show an enlarged spleen. |
|
Animal health report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Rapgef6 |
| Symbol name |
Rap guanine nucleotide exchange factor (GEF) 6 |
| Chromosome |
11 |
| Common name |
C030018K18Rik, PDZ-GEF2, Pdzgef2, RA-GEF-2 |
| Symbol description |
No Data |
| 2 |
Symbol |
loxP |
| Symbol name |
phage P1 loxP |
| Chromosome |
11 |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
Mol Biol Cell. 2007 May 30;. The M-Ras-RA-GEF-2-Rap1 Pathway Mediates Tumor Necrosis Factor-{alpha}-dependent Regulation of Integrin Activation in Splenocytes. |
|
Research applications |
Cell Biology Research,
Immunology and Inflammation Research |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Biol. Cell, 18, 2949-2959 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE. |
|
Additional information |
|
1 |
Lab HP (Japanese) |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Kataoka, Tohru (Kobe University)  |
|
Strain Status /
Availability
(Expected delivery) |
|
