Strain Data Sheet

RBRC02839

Strain Information

Image
BRC No.RBRC02839
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-GFP/DA)43
Former Common nameGFP-DATg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Tomoaki Shirao, Gunma University School of Medicine in 2001.
Strain descriptionDrebrin is an F-actin-binding protein that is highly expressed in the brain and regulates the dynamics of actin cytoskeletal networks. These mice specifically overexpress a fusion protein of rat drebrin A and GFP in the forebrain. A western blot analysis of cerebral cortex extract revealed a 10 to 15% increase in drebrin (similar expression patterns were observed in three lines: RBRC02838, RBRC02839, RBRC0284015). These mice are useful for analyzing the effect of the overexpression of drebrin at the cellular and body levels.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dbn1drebrin 1 (rat)UNDbn1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Camk2a promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAmouse Camk2a genomic DNA, jellyfish GFP cDNA, rat drebrin A cDNA, SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR/DEVELOPER to obtain permission for use o the BIOLOGICAL RESOURCE as a collaboration research.
DepositorTomoaki Shirao (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data