Strain Information | |
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Image | |
BRC No. | RBRC02868 |
Type | Transgene Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tg(CAT-RhoK DN)4-11Koba |
Former Common name | CAT-RhoA-K DN/4-11 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine at 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. |
Strain description | Transgenic mice conditionally expressed a dominant-negative form for RhoK by using the Cre-loxP system. The Rho GTPase is known to regulate diverse cellular processes, including cell morphology, cell motility, adhesion, and apoptosis. The activity of RhoK in these transgenic mice is suppressed in developing motor neurons by crossing with dopamine beta-hydroxylase promotor (DBH)-cre transgenic mice. This strain are useful for elucidation of the role of the Rho sigaling pathway in various tissues with tissue-specific cre Tg mice. |
Colony maintenance | |
References | J. Neurosci., 24, 3480-3488 (2004). 15071095 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rhokras homolog gene family, member A (bovine)UNRhok Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)UNE. coliCMV promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter myc-tagmyc-epitope tagUNmyc-tag Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ASV40 polyA signalUNpoly ASV40 |
Ordering Information | |
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Donor DNA | Cytomegalovirus CMV promoter, E. coli Chloramphenicol acetyl transferase, bovine Rho-kinase, SV40 polyA signal |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 24, 3480-3488 (2004). |
Depositor | Kazuto Kobayashi (Fukushima Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |