Strain Data Sheet

RBRC02884

Strain Information

Image
BRC No.RBRC02884
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB/N-Tg(Pitx3-cre/ESR1)1Yqd
Former Common nameTg(Pitx3-cre/ESR1)1Ydq; FVB/N-Tg(Pitx3-cre/ESR1)1Yqd
H-2 Haplotype
ES Cell line
Background strainFVB/N
Appearance
Strain developmentThe transgene was microinjected into FVB fertilized eggs, then later was crossed to C57BL/6 for two generations.
Strain descriptionViable and fertile with no obvious abnormalities.
Colony maintenanceFVB/N-Tg(Pitx3-cre/ESR1)1Ydq transgenic mice was made by inserting CreER into the Pitx3 ATG locus of a mouse BAC clone.
ReferencesGenesis, 46., 324-328 (2008). 18543300

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor 1 (alpha)UNERT2transgene insertion 1, Yu-qiang Ding

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse Pitx3 promoter

Ordering Information

Donor DNAmouse Pitx3 promoter, Phage P1 cre recombinase gene, human estrogen receptor 1 cDNA, bovine growth hormone polyA, yeast FRT (flipase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 46, 324-328 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYu Qiang Ding (Chinese Academy of Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data