Strain Data Sheet

RBRC02900

Strain Information

Image
BRC No.RBRC02900
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Poll<tm1Nmt>
Former Common namePOLL KO mice, DNA polymerase lamda KO mouse
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Y. Kobayashi, K. Ikeda and N. Motoyama in National Institute for Longevity Sicences at 2000.
Strain descriptionPoll, polymerase (DNA directed), lambda gene knock out mice. Homozygous mutant mice show hydrocephalus and a high rate of mortality after birth. The surviving males are sterile due to spermatozoal immobility.
Colony maintenanceHeterozygote x Wild-type (C57BL/6JJcl)
ReferencesMol. Cell. Biol., 22, 2769-2776 (2002). 11909969

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pollpolymerase (DNA directed), lambda19Poll<tm1Nmt>targeted mutation 1, Noboru Motoyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse PGK (phosphoglycerate kinase) promoter, Mouse PGK polyA (phosphoglycerate kinase gene terminater), E.coli Neomycin resistance gene, phage P1 loxP, Mouse polymerse (DNA directed), lambda genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 22, 2769-2776 (2002).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Motoyama (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data