Strain Data Sheet

RBRC02945

Strain Information

Image
BRC No.RBRC02945
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Thy1-ECFP,-EYFP)1Hsak
Former Common nameThy1-stop-syn::ECFP-ires-gEYFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ko Kobayakawa, Reiko Hayashi and Hitoshi Sakano, School of Science The University of Tokyo in 2002. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThy1-stop-syn::CFP-ires-gapEYFP mice having a transgene of a Thy1 promoter, loxP-STOP loxP cassette, synaptotagmin-CFP and ires-gapEYFP. Tissue-specific expression of Cre recombinase has not been examined yet.
Colony maintenanceCarrier x Noncarrier [or Crossing to Crlj:CD1(ICR)]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria)UNECFPmouse Thy1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gap43mouse gap43 palmitoylation signalUNGap43

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
His3His3 (S. cerevisiae)UNHis3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse synaptotagmin I (Syt1)UN

Ordering Information

Donor DNAMouse Thy1 promoter, P1 phage loxP site, S. cerevisiae His3, Jellyfish ECFP, EYFP, encephalomyocarditis virus ires (internal ribosome entry site, Mouse gap43 palmitoylation signal, Mouse synaptotagmin I (Syt1)
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER (Ko KOBAYAKAWA : skobayak@mail.ecc.u-tokyo.ac.jp). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
DepositorHitoshi Sakano (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data