Strain Data Sheet
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Data update: Sep 21, 2017
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC02975  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129S-Atg5<tm1Myok>  
Former Common name Atg5flox  
H-2 Haplotype No Data  
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Kenji Nakamura, Mitsubishi Kagaku Institute of Life Sciences and Noboru Mizushima, Tokyo Metropolitan Institute of Medical Science in 2003. The construct was electoroporated into CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c>. The mutant mice were backcrossed to C57BL/6J.  
Strain description Atg5 floxed mice. Exon 3 of Atg5 gene was flanked by loxP sites containing neomycin resistant cassette. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional B6.129S-Atg5<tm1Myok> (RBRC02975) mice can be crossed with various tissue-specific Cre transgenic mice to study the role of autophagy in adult tissues. Null knockout mice are also available: B6;129-Atg5<tm1Nmz> (RBRC02231).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Atg5  
Symbol name autophagy-related 5 (yeast)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 10  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name neo; neomycin;  
Symbol description No Data  
References Nature. 2006 Jun 15;441(7095):885-9. Epub 2006 Apr 19.  
Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 441, 885-889 (2006).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Mouse of the Month Jul 2009
Genetic Background
Breeding characters  
 2 Lab HP  
 3 Genotyping protocol <PCR>  
Depositor Mizushima, Noboru (The University of Tokyo, Graduate School of Medicine)  Mizushima, Noboru 
Strain Status /
Availability
(Expected delivery)

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