Strain Data Sheet

RBRC03325

Strain Information

Image
BRC No.RBRC03325
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Btbd3<tm1Seto>/SetoRbrc
Former Common nameBtb-D3 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tomoaki Kahyo and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences.
Strain descriptionBtb-D3 (Btbd3) gene knockout mice. Exons 1 and 2 were replaced with a neomycin resistant cassette. Homozygous mutant mice exhibit a fertility decline and a low body weight.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Btbd3BTB (POZ) domain containing 32Btbd3<tm1Seto>targeted mutation 1, Mitsutoshi Setou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;

Ordering Information

Donor DNABtbd3 genomic DNA (mouse), Neomycin resistance gene (E. coli)
Research application
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorMitsutoshi Setou (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data