Strain Information | |
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Image | |
BRC No. | RBRC03515 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Aire<tm2Mmat>/Rbrc |
Former Common name | Aire(GFP) (Acc.No. CDB0479K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used. |
Strain description | Aire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2. |
Colony maintenance | |
References | J. Exp. Med., 205, 2827-2838 (2008). 19015306 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Aireautoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)10Aire<tm2Mmat>targeted mutation 2, Mitsuru Matsumoto Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neo, jellyfish GFP cDNA, mouse Aire genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |