Strain Data Sheet
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Data update: Aug 27, 2014
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RBRC No. RBRC03666  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name Arx-6 KO/GAD67 GFPdNeo KI  
Former Common name No Data  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences.  
Strain description Arx gene knockout and GAD67-GFP (Gad1-GFPdNeo) double mutant mice. Arx: A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. GAD67: An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are rethal. A loxP flanked neo was removed by crossing with cre mice.  
Colony maintenance Arx: Heterozygote (female) x Wild-type (male); Gad1: Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]  
Health Certificate No Data  
Gene Details
Promoter No Data  
 1 Symbol Gad1  
Symbol name glutamic acid decarboxylase 1  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 4 Symbol Arx  
Symbol name aristaless related homeobox  
Chromosome X  
Common name No Data  
Symbol description No Data  
 5 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 6 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 7 Symbol neo  
Symbol name No Data  
Chromosome X  
Common name No Data  
Symbol description No Data  
 8 Symbol No Data  
Symbol name SV40 PolyA signal  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
References Nat Genet 2002 Nov;32(3):359-69. Epub 2002 Oct 15.  
Mutation of ARX causes abnormal development of forebrain and testes in mice and X-linked lissencephaly with abnormal genitalia in humans.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation)  Kitamura, Kunio 
Strain Status /
Availability
(Expected delivery)

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