Strain Information | |
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Image | |
BRC No. | RBRC03674 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Gad1<tm1.1Tama> |
Former Common name | GAD67-GFP dNeo KI |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background. |
Strain description | An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)] |
References | J. Comp. Neurol., 467, 60-79 (2003). 14574680 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-BRack:D 2023/11/27Room:4-BRack:D 2023/08/28Room:4-BRack:D 2023/05/29Room:4-BRack:D 2023/04/10Room:4-BRack:D 2023/02/27Room:4-BRack:D 2022/11/29Room:4-BRack:D 2022/08/29Room:4-BRack:D 2022/05/31Room:4-BRack:D |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | Jellyfish green fluorescent protein cDNA, phage P1loxP site, mouse Gad1 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp Neurol., 467, 60-79 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Yuchio Yanagawa (Gunma University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jan 2014 |
BRC mice in Publications |
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Jahan MS, Ito T, Ichihashi S, Masuda T, Bhuiyan MER, Takahashi I, Takamatsu H, Kumanogoh A, Tsuzuki T, Negishi T, Yukawa K. PlexinA1 deficiency in BALB/cAJ mice leads to excessive self-grooming and reduced prepulse inhibition. IBRO Rep 9 276-289(2020) 33163687 Saito K, Koike T, Kawashima F, Kurata H, Shibuya T, Satoh T, Hata Y, Yamada H, Mori T. Identification of NeuN immunopositive cells in the adult mouse subventricular zone. J Comp Neurol 526(12) 1927-1942(2018) 29752725 Carstens KE, Phillips ML, Pozzo-Miller L, Weinberg RJ, Dudek SM. Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons. J Neurosci 36(23) 6312-20(2016) 27277807 Fu X, Teboul E, Weiss GL, Antonoudiou P, Borkar CD, Fadok JP, Maguire J, Tasker JG. Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice. Nat Commun 13(1) 1290(2022) 35277502 |