Strain Data Sheet
RBRC03730
Strain Information | |
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| Image | |
| BRC No. | RBRC03730 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Dnmt3a<tm1Enl> |
| Former Common name | Dnmt3a<tm1Enl>; Dnmt3a null KO |
| H-2 Haplotype | |
| ES Cell line | J1 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masaki Okano and En Li, Massachusetts General Hospital in 1999. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6. |
| Strain description | Dnmt3a knockout mice. A 0.3 kb region containing exons of Dnmt3a encoding PC and ENV motif was replaced with an IRES-beta-geo cassette. Homozygous mutant mice appear normal at birth, but most mutant mice become runted and die at about 4 week of age. Dnmt3a floxed mice (RBRC03731), Dnmt3b knockout mice (RBRC03732), Dnmt3b floxed mice (RBRC03733). |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Cell, 99, 247-257 (1999). 10555141 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12neo; neomycin; |
Ordering Information | |
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| Donor DNA | mouse En-2 splice acceptor, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, E. coli neomycin registance gene, SV40 poly A signal sequence, mouse Dnmt3a genomic DNA |
| Research application | |
| Specific Term and Conditions | A. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Cell 1999 99:247-57 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient. |
| Depositor | Masaki Okano (RIKEN CDB/ MGH) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Breeding characters |
BRC mice in Publications |
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Yagi M, Kabata M, Tanaka A, Ukai T, Ohta S, Nakabayashi K, Shimizu M, Hata K, Meissner A, Yamamoto T, Yamada Y. Identification of distinct loci for de novo DNA methylation by DNMT3A and DNMT3B during mammalian development. Nat Commun 11(1) 3199(2020) 32581223 |