Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Apr 24, 2017
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RBRC No. RBRC03733  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S4-Dnmt3b<tm5.1Enl>  
Former Common name Dnmt3b; Dnmt3b conditional KO, Dnmt3b-2lox  
H-2 Haplotype No Data  
ES cell line J1 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Masaki Okano and En Li, Massachusetts General Hospital in 2001. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6.  
Strain description Dnmt3b floxed mice. Exon 19 of the Enmt3b was flanked by loxP sites. Conditional Dnmt3a deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Dnmt3a knockout mice (RBRC03730), Dnmt3a floxed mice (RBRC03731), Dnmt3b knockout mice (RBRC03732).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Dnmt3b  
Symbol name DNA methyltransferase 3B  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 2  
Common name No Data  
Symbol description No Data  
References J Biol Chem. 2005 May 6;280(18):17986-91. Epub 2005 Mar 8.  
Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization.
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
A. RECIPIENT must send a copy of the executed MTA for distribution between RECIPIENT and RIKEN BRC to Massachusetts General Hospital's Research and Licensing Office: 101 Huntington Ave, 4th Floor, Boston, MA 02215, (617)954-9500.
B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs.
C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature J Biol Chem. 2005 280:17986-91 as designated by the DEPOSITOR is required.
D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes.
E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office.
F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.  
Additional information
 1 Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).  
 2 Genotyping protocol <PCR>  
Depositor Okano, Masaki (RIKEN)  Okano, Masaki 
Strain Status /
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