Strain Information | |
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Image | |
BRC No. | RBRC04292 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6-Pold1<tm1.uchi> |
Former Common name | Pold1<tm1Uchi> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The mutant mice were crossed to C57BL/6J. |
Strain description | Pold1 (polymerase (DNA directed), delta 1, catalytic subunit) gene knockout mice. A loxP flanked polyA-neo cassette was inserted in intron 10. The exon 10 contains a D400A substitution. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
References | PLoS One, 4, e4184 (2009). 19145245 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pold1polymerase (DNA directed), delta 1, catalytic subunit7Pold1<tm1Uchi>targeted mutation 1, Arikuni Uchimura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal7 |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), SV40 PolyA signal, P1 phage loxP, E. coli neo, mouse Pold1 genomic DNA |
Research application | Cell Biology Research Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takeshi Yagi (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |