Strain Data Sheet

RBRC04415

Strain Information

Image
BRC No.RBRC04415
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Lefty1-Cre/ERT2)8Hmd
Former Common nameLefty1-CreERT2/BAC (8)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kastuyoshi Takaoka and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009.
Strain description
Colony maintenance
ReferencesNat. Cell Biol., 13, 743-752 (2011). 21623358

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse lefty1 promoter

Ordering Information

Donor DNAmouse Lefty1 genomic DNA from BAC clone RP23-390I1, Phage P1 Cre, human Estrogen receptor binding domain (ERT2) cDNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Cell Biol., 13, 743-752 (2011).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Tsubosaka A, Komura D, Kakiuchi M, Katoh H, Onoyama T, Yamamoto A, Abe H, Seto Y, Ushiku T, Ishikawa S.
Stomach encyclopedia: Combined single-cell and spatial transcriptomics reveal cell diversity and homeostatic regulation of human stomach.
Cell Rep 42(10) 113236(2023) 37819756