Strain Data Sheet
RBRC04421
Strain Information | |
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| Image | |
| BRC No. | RBRC04421 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129(FVB)-Tcirg1<tm1.2Ywd> |
| Former Common name | a3 mutant, Tcirg1<delta> |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008. |
| Strain description | The gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. Homozygous mutant mice die within 3-4 weeks after birth. |
| Colony maintenance | |
| References | J. Cell Sci., 122, 2504-2513 (2009). 19549681 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.2Ywd>targeted mutation 1.2, Yoh Wada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP |
Ordering Information | |
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| Donor DNA | Phage phage P1 loxP site, Mouse Tcirg1 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Yoh Wada (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Matsumoto N, Nakanishi-Matsui M. Proton pumping V-ATPase inhibitor bafilomycin A1 affects Rab7 lysosomal localization and abolishes anterograde trafficking of osteoclast secretory lysosomes. Biochem Biophys Res Commun 510(3) 421-426(2019) 30717974 Matsumoto N, Sekiya M, Sun-Wada GH, Wada Y, Nakanishi-Matsui M. The lysosomal V-ATPase a3 subunit is involved in localization of Mon1-Ccz1, the GEF for Rab7, to secretory lysosomes in osteoclasts. Sci Rep 12(1) 8455(2022) 35589873 |