Strain Data Sheet

RBRC04488

Strain Information

Image
BRC No.RBRC04488
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129P2(B6)-Sirpa<tm1Nog>
Former Common nameSIRP alpha mutant mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Kenjiro Inagaki at Kobe University in 2000. A neomycin selection cassette replaced exons 7 and 8 of the M83 gene. The mutant mice were backcrossed C57BL/6N for 5 times, and then backcrossed to BALB/c over 15 times.
Strain descriptionSHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene is a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenance
ReferencesJ. Biol. Chem., 277 (42) 39833-9 (2002). 12167615
EMBO J., 19, 6721-6731 (2000). 11118207

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sirpasignal-regulatory protein alpha2Sirpa<tm1Nog>targeted mutation 1, Tetsuya NoguchiBit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, PGK PolyA, mouse Sirpa genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT f BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
DepositorTakashi Matozaki (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data