Strain Information | |
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Image | |
BRC No. | RBRC04707 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | STOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama> |
Former Common name | Kv3.GYW |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Thomas Knoepfel, RIKEN Brain Science Institute. |
Strain description | Kcnc1-YFP transgenic and GAD67 GFP knock-in double mice. Transgene composed of 5kB 5’ regulatory sequence of rat Kv3.1 potassium channel driving expression of a yellow variant of jelly fish green fluorescent protein. Knock-in of GFP into the ATG in exon 1 of GAD67 gene. Bright yellow and green fluorescence of specific cell types. GAD67 GFP homozygous mice are infertile. |
Colony maintenance | Tg : Carrier x Carrier; KO : Heterozygote x Wild-type |
References | J. Comp. Neurol., 467, 60-79 (2003). 14574680 Eur. J. Neurosci., 15, 40-50 (2002). 11860505 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFPrat Kcnc1 promoter |
Ordering Information | |
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Donor DNA | rat Kv3. 1 (Kcnc1) genomic DNA, Jellyfish EYFP cDNA, Simian virus SV40 polyA, mouse GAD67 genimic DNA, Jellyfish GFP cDNA, Phage P1 loxP, [Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene ] |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | BEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR: a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form. b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. c. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50., J Comp Neurol. 2003 467(1):60-79. AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR: a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced). b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Thomas Knöepfel (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |