Strain Data Sheet

RBRC04719

Strain Information

Image
BRC No.RBRC04719
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Cluap1<tm1.1Hmd>
Former Common nameQilin (null/+)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Yanick Botlde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6/129. Correctly targeted ES cells were aggregated with ICR molura. The resulting chimeric animals were crossed to C57BL/6 and then crossed to CAG-fLPe and CAG-Cre mice.
Strain descriptionCluap1 (Qilin) gene knockout mice. Exons 1 to 3 were removed. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev. Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cluap1clusterin associated protein 116Cluap1<tm1.1Hmd>targeted mutation 1.1, Hiroshi HamadaQilin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNAbacteriophage由来 loxP配列, yeast由来 frt配列, chicken/rabbit/Cytomegalovirus由来 CAGプロモーター, yeast由来 flpe遺伝子
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 381(1), 203-212 (2013).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data