Strain Information | |
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Image | |
BRC No. | RBRC04719 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129B6-Cluap1<tm1.1Hmd> |
Former Common name | Qilin (null/+) |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yanick Botlde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6/129. Correctly targeted ES cells were aggregated with ICR molura. The resulting chimeric animals were crossed to C57BL/6 and then crossed to CAG-fLPe and CAG-Cre mice. |
Strain description | Cluap1 (Qilin) gene knockout mice. Exons 1 to 3 were removed. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Dev. Biol., 381(1), 203-212 (2013). 23742838 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP |
Ordering Information | |
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Donor DNA | bacteriophage由来 loxP配列, yeast由来 frt配列, chicken/rabbit/Cytomegalovirus由来 CAGプロモーター, yeast由来 flpe遺伝子 |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 381(1), 203-212 (2013). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |