Strain Data Sheet

RBRC04773

Strain Information

Image
BRC No.RBRC04773
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR;B6C3-Tg(CAG-cat,-HTF/Nanos2)7
Former Common nameHTF-nanos2 #7
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009.
Strain description
Colony maintenance
ReferencesGenes Dev., 22, 430-435 (2008). 18281459
Science, 325, 1394-1398 (2009). 19745153

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nanos2nanos homolog 2 (Drosophila)UNNanos2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TEVTobacco Etch Virus (TEV) proteaseUNTEV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNE. coliCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, mouse Nanos2 gene cDNA, human influenza virus Ha (hemagglutinin) tag cDNA, Tobacco Etch Virus (TEV) protease
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from DEPOSITOR and Prof. Yumiko Saga, National Institute of Genetics. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and /or the DEVELOPER's names as co-authors.
DepositorAtsushi Suzuki (Yokohama National University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data