Strain Data Sheet
RBRC04790
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC04790 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Capn9<tm1Hiso> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NJcl |
| Appearance | |
| Strain development | Developed by Drs. Shoji Hata and Hiroyuki Sorimachi, The Tokyo Metropolitan Institute of Medical Science Rinshoken in 2007. Exon 3 of the Capn9 gene was replaced with a neomycin resistant cassette. C57BL/6 background. |
| Strain description | |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
| References | PLoS Genet., 6, e1001040 (2010). 20686710 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ApolyA8poly A |
Ordering Information | |
|---|---|
| Donor DNA | bacteriophage P1 LoxP sites, yeast FRT sites, mouse pgk promoter, E. coli Neomycin resistance gene, mouse pgk polyA signal, mouse Capn9 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS Genet. 2010 6(7):e1001040. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research which is premised on publication. 4) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues are included as co-authors, who are designated by the DEPOSITOR. |
| Depositor | Hiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |