Strain Data Sheet

RBRC04800

Strain Information

Image
BRC No.RBRC04800
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-EpCAM<tm1.2Hmd>
Former Common nameEpCAM KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6129F1. The Exon2 and exon3 was excised by crossing with CAG-Cre mice.
Strain descriptionTacstd1 (Epcam, epithelial cell adhesion molecule) gene knockout mice. Exon2 and exon3 of Tacstd1 was deleted by crossing with CAG-Cre mice. Homozygous mice die within 10 days after birth.
Colony maintenanceHeterozygote X Wild-type [C57BL/6JJcl]
ReferencesDev. Biol., 371, 136-145 (2012). 22819673

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Epcamepithelial cell adhesion molecule17Epcamepithelial cell adhesion molecule

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Ordering Information

Donor DNAbacteriophage由来 loxP配列
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 371, 136-145 (2012).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data