Strain Information | |
---|---|
Image | |
BRC No. | RBRC04801 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Kcp<tm1Ysas>/Rbrc |
Former Common name | Kcp(tau-lacZ) (Acc.No.CDB0401K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Yoshiki Sasai, Center for Developmental Biology, RIKEN. |
Strain description | This transgene contains tau-lacZ with a floxed neo in the first exon of Kcp to replace with lacZ at the first ATG. Kcp is a Kielin-related molecule that contains multiple Chd-type cysteine-rich repeats, a vWD domain and a TIL domain, and physically binds to Bmp proteins. Homozygous mutant mice are viable and fertile, and exhibit no gross structural defects. |
Colony maintenance | Heterozygote X Wild-type [C56BL/6] Homozygous mutant mice are viable and fertile. |
References | Development, 133, 4463-4473 (2006). 17035289 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ASV40 polyA6poly A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter taubovine tau protein cDNA6tau |
Ordering Information | |
---|---|
Donor DNA | bovine tau cDNA, E. coli lacZ, SV40 polyA, phage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Kcp genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from Dr. Makoto Ikeya (mikeya@cira.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0401K, and Web Site (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Development, 133, 4463-4473 (2006).) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |