Strain Information | |
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Image | |
BRC No. | RBRC04803 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Bmper<tm2Ysas>/Rbrc |
Former Common name | Cv2(nlacZ) (Acc.No.CDB0451K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Yoshiki Sasai, Center for Developmental Biology, RIKEN. |
Strain description | This transgene contains beta-galactosidase with a nuclear localization signal. The first methionine of Cv2 was replaced in-flame with that of lacZ. Cv2 (Bmper : BMP-binding endothelial regulator) is essential for multiple organogenetic processes that involve Bmp signals. Cv2 homozygous mutant mice show effects in the vertebral column and eyes. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6] Homozygous mutant mice are neonatal lethal. |
References | Development, 133, 4463-4473 (2006). 17035289 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9neo; neomycin; |
Ordering Information | |
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Donor DNA | LacZ (大腸菌), polyA (SV40), PGK promoter (mouse) Neo (大腸菌), loxP (P1ファージ) |
Research application | |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from Dr. Makoto Ikeya (mikeya@cira.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0451K, and Web Site (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Development, 133, 4463-4473 (2006).) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |