Strain Information | |
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Image | |
BRC No. | RBRC04807 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Spata9<tm1Osb> |
Former Common name | B6-Spata9 KO |
H-2 Haplotype | |
ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | Developed by Hide Hatayama and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2010. |
Strain description | Spata9 gene knockout mice. spata9, spermatogenesis associated 9 is a gene specifically expressing in testis. |
Colony maintenance | Heterozygote X Wild-type [C57BL/6NJcl] |
References | Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Spata9spermatogenesis associated 913Spata9<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site13Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site13Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP13loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK pA signal, phage P1 loxP site, yeast FRT (flipase recombination target) sites, mouse Spata9 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |