Strain Information | |
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Image | |
BRC No. | RBRC04841 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6N.129P2-Bhlhe40<tm1Homy> |
Former Common name | Dec1 KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The promoter and coding region including the ATG in exon1 was replaced with a neomycin resistance gene. C57BL/6N background |
Strain description | Dec1 (Bhlhe40) knockout mice. The promoter and coding region including the ATG in exon 1 was replaced with a neomycin resistance gene. Homozygous mutant mice exhibit a decrease in T cell number in aged mice and a development of lymphoproliferative autoimmune disease. |
Colony maintenance | |
References | J. Immunol., 185, 7330-7339 (2010). 21057086 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (Streptomyces fradiase)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), Streptomyces fradiase neomycin phosphotransferase genomic DNA, mouse Bhlhe40 genomic DNA |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Hu J, Mao Z, He S, Zhan Y, Ning R, Liu W, Yan B, Yang J. Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway. Biochem Pharmacol 136 109-121(2017) 28408345 Wu Y, Wang H, Huo Y, Yan B, Honda H, Liu W, Yang J. DIFFERENTIATED EMBRYONIC CHONDROCYTE EXPRESSED GENE-1 IS A CENTRAL SIGNALING COMPONENT IN THE DEVELOPMENT OF COLLAGEN-INDUCED RHEUMATOID ARTHRITIS. J Biol Chem 102982(2023) 36739947 Hirata H, Kamohara A, Murayama M, Nishioka K, Honda H, Urano Y, Soejima H, Oki S, Kukita T, Kawano S, Mawatari M, Kukita A. A novel role of helix-loop-helix transcriptional factor Bhlhe40 in osteoclast activation. J Cell Physiol 237(10) 3912-3926(2022) 35908202 Hu X, Zou M, Zheng W, Zhu M, Hou Q, Gao H, Zhang X, Liu Y, Cheng Z. Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis. Respir Res 25(1) 100(2024) 38402153 |