Strain Data Sheet

RBRC04850

Strain Information

Image
BRC No.RBRC04850
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(NDE-hsp-Pkd2*R6GdC/Venus)101Hmd
Former Common nameNDE-Pkd2ml(delta)C-Venus(101)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Satoko Yoshiba and Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010 The transgene was injected into the pronuclei of (C57BL/6 x C3H) F1 fertelized eggs. C57BL/6, C3H and 129 mized background.
Strain descriptionThese transgenic mice express the Arg6Gly variant Pkd2 and venus on both nude under the control of the node specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesScience, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkd2polycystic kidney disease 2UNPkd2mouse node-specific enhancer, hsp promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusVenus

Ordering Information

Donor DNAマウス由来Nodalのノードエンハンサー, 変異型Pkd2cDNA, hspプロモーター, 改変型クラゲ由来EGFP
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data