Strain Data Sheet

RBRC04852

Strain Information

Image
BRC No.RBRC04852
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3(129)-Tg(NDE-hsp-Smad3DN,lacZ)1Hmd
Former Common nameNDE-dnSmad3-1Z(1)
H-2 Haplotype
ES Cell line
Background strainJcl:B6C3F1
Appearance
Strain developmentDeveloped by Aiko Kawasumi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009 The transgene was injected into the pronuclei of (C57BL/6 x C3H) F1 fertelized eggs. C57BL/6, C3H and 129 mixed background.
Strain description
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6C3F1]
ReferencesDev. Biol., 353, 321-330 (2011). 21419113

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Smad3SMAD family member 3UNSmad3mouse hsp promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Ordering Information

Donor DNAマウス由来NodalのNDEエンハンサー dominant negative (3’ AAVA) 型に改変したSmad3 cDNA, hspプロモーター, E. coli由来LacZ遺伝子, ウィルス由来IRES配列
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 353, 321-330 (2011).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data