Strain Data Sheet

RBRC04874

Strain Information

Image
BRC No.RBRC04874
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineB6N-22<UTR> [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center in 2011. B6N-22<Ttr> ES cells were used. C57BL/6N background.
Strain descriptionR26GRR knock-in mice. A construct consisting of the EGFP-tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter, was inserted via homologous recombination into the intron between exons1 and 2 of the ROSA26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrl]
ReferencesExp. Anim., 71(3), 385-390 (2022). 35444103
BMC Res. Notes 16(1) 54 (2023). 37069662
BMC Res. Notes 16(1) 109 (2023). 37337280
Exp. Anim., 62, 295-304 (2013). 24172193

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:F
2023/10/10Room:3-BRack:F
2023/07/10Room:3-BRack:F
2023/04/10Room:3-BRack:F
2023/01/10Room:3-BRack:F
2022/10/11Room:3-BRack:F
2022/07/11Room:3-BRack:F
2022/04/11Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor<tm1(CAG-EGFP/DsRed)Utr>targeted mutation 1 (CAG-EGFP/DsRed), University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tDsRedRed Fluorescent Protein (Discosoma sp.)6tDsRed

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

Donor DNARosa gene (ubiquitous) (mouse), CMV IE enhancer (cytomegalovirus), beta-actin promoter (chicken), beta-globin genomic DNA (rabbit), EGFP (jellyfish), DsRed (red flourescent protein from Discosoma sp.) cDNA, PGK promoter (mouse), neomycin resistance gene (E. coli), polyA additional signal (mouse), FRT sites (yeast), loxP sites (P1 phage), phage T7 gb2 promoter(synthetic), polyA (SV40), FLAG tag (synthetic)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim 62, 295-304 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Mar 2014, Mouse of the Month Oct 2018
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Hashimoto M, Sasaki H.
Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells.
Dev Cell 50(2) 139-154.e5(2019) 31204175

Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H.
AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High-Fat Diet.
Endocrinology 157(4) 1457-66(2016) 26889940

Matsumoto L, Hirota Y, Saito-Fujita T, Takeda N, Tanaka T, Hiraoka T, Akaeda S, Fujita H, Shimizu-Hirota R, Igaue S, Matsuo M, Haraguchi H, Saito-Kanatani M, Fujii T, Osuga Y.
HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment.
J Clin Invest 128(7) 3186-3197(2018) 29911998

Hiraoka T, Hirota Y, Saito-Fujita T, Matsuo M, Egashira M, Matsumoto L, Haraguchi H, Dey SK, Furukawa KS, Fujii T, Osuga Y.
STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation.
JCI Insight 1(8) (2016) 27358915

Mizoguchi A, Banno R, Sun R, Yaginuma H, Taki K, Kobayashi T, Sugiyama M, Tsunekawa T, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Suga H, Nagai T, Yamada K, Arima H.
Glucocorticoid receptor signaling in ventral tegmental area neurons increases the rewarding value of a high-fat diet in mice.
Sci Rep 11(1) 12873(2021) 34145364

Tsunekawa T, Banno R, Yaginuma H, Taki K, Mizoguchi A, Sugiyama M, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Bettler B, Arima H.
GABAB Receptor Signaling in the Mesolimbic System Suppresses Binge-like Consumption of a High-Fat Diet.
iScience 20 337-347(2019) 31610370