Strain Information | |
---|---|
Image | |
BRC No. | RBRC04878 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;129-Tg(CMV-EGFP,U6-shNanog)1Ttada |
Former Common name | NRi-Tg |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Shinpei Yamaguchi and Takashi Tada, Institute for Frontier Medical Sciences, Kyoto University in 2007. |
Strain description | |
Colony maintenance | Carrier x Carrier |
References | Development, 136, 4011-4020 (2009). 19906868 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCMV promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TATAloxphage P1 loxP (modified loxP site)UNTATAlox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TATAloxphage P1 loxP (modified loxP site)UNTATAlox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE |
Ordering Information | |
---|---|
Donor DNA | 5' LTR, HIV PRE, FLAP, mouse U6 promoter, Phage P1 TATAlox sites (modified loxP site), Cytomegalovirus CMV promoter, jellyfish EGFP cDNA, Nanog shRNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), 3'SIN LTR [update April 12, 2019] |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Takashi Tada (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K. NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism. Cell Metab 23(1) 206-19(2016) 26724859 |