Strain Data Sheet

RBRC04906

Strain Information

Image
BRC No.RBRC04906
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameD1.129P2(Cg)-Cxcr4<tm1Yiw> Tg(Lck-cre)1Jtak
Former Common nameD1-Cxcr4 floxed/LckCre Tg
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentCxcr4 floxed mice were developed by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. Lck-cre Tg mice were developed by Junji Takeda, Osaka University in 1998. Double mutant mice were backcrossed DBA/1 for at least 8 times.
Strain descriptionCxcr4 floxed mice with Lck-cre transgene.
Colony maintenanceDBA/1JJmsSlc
ReferencesArthritis Res. Ther., 12, R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcr4chemokine (C-X-C motif) receptor 41Cxcr4<tm1Yiw>targeted mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse lck promoter

Ordering Information

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cxcr4 genomic DNA, mouse Lck promoter, phage P1 Cre recombinase gene
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Res. Ther., 12, R188 (2010). Eur. J. Immunol., 28(7):2159-2166 (1998).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data