Strain Data Sheet

RBRC04926

Strain Information

Image
BRC No.RBRC04926
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Epha2<Gt(ROSABetageo)CN3Yiw>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2001. The mice were backcrossed to C57BL/6 for at least 8 times.
Strain descriptionEph receptor 2 knockout mice. Tail abnormality are frequently observed.
Colony maintenance
ReferencesMech. Dev., 102, 95-105 (2001). 11287184

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Epha2Eph receptor A24Epha2<Gt(ROSABetageo)CN3Yiw>gene trap CN3, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
LTRMoMuLV long terminal repeat4LTR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor4SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)4nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Apolyadenylic acid additional signal4poly A

Ordering Information

Donor DNALTR (Mo-MuLV), Splice acceptor, Simian virus 40 Large T antigen nuclear localization signal, E. coli beta geo (Neomycin resistance gene, lacZ), polyadenylic acid additional signal, mouse Epha2 genomic DNA [update Feb 28, 2014]
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech. Dev., 102, 95-105 (2001).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data