|
RBRC No. |
RBRC04949
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
B6.B6CB(Cg)-Psmd14<tm1.1Smta> |
|
Former Common name |
B6-Psmd14/FlagGFP KI (Rpn11-Flag/GFP) |
|
H-2 Haplotype |
No Data |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
No Data |
| Genotype |
No Data |
|
Strain development |
Developed by Shigeo Murata, The Tokyo Metropolitan Institute of Medical Sicence in 2002. |
|
Strain description |
No Data |
|
Colony maintenance |
Heterozygote x Wild-type [C57BL/6JJmsSlc] |
|
Animal health report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
GFP |
| Symbol name |
Green Fluorescent Protein (Jellyfish) |
| Chromosome |
2 |
| Common name |
EGFP; Egfp, enhanced green fluorescent protein; |
| Symbol description |
No Data |
| 2 |
Symbol |
Psmd14 |
| Symbol name |
proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 |
| Chromosome |
2 |
| Common name |
No Data |
| Symbol description |
No Data |
| 3 |
Symbol |
FLAG |
| Symbol name |
FLAG tag (synthetic) |
| Chromosome |
2 |
| Common name |
No Data |
| Symbol description |
No Data |
| 4 |
Symbol |
His |
| Symbol name |
His tag (synthetic) |
| Chromosome |
2 |
| Common name |
No Data |
| Symbol description |
No Data |
| 5 |
Symbol |
loxP |
| Symbol name |
phage P1 loxP |
| Chromosome |
2 |
| Common name |
No Data |
| Symbol description |
No Data |
| 6 |
Symbol |
frt |
| Symbol name |
yeast FRT (flippase recombination target) sites |
| Chromosome |
No Data |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
|
|
Research applications |
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The recipient must obtain a prior written consent on use of it from the depositor. In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only. |
|
Additional information |
|
1 |
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Murata, Shigeo (The University of Tokyo)  |
|
Strain Status /
Availability
(Expected delivery) |
|
