Strain Information | |
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Image | |
BRC No. | RBRC05198 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Ciz1<tm1Homy> |
Former Common name | Ciz1 cKO B6-Ciz1 floxed |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2008. A loxP was inserted upstream of exon 5 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 6 times. |
Strain description | Ciz1 gene floxed mice. Exon 5 of Ciz1 gene is flanked by loxP sites. Conditonal Ciz1 deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Heterozygote X Wild-type [C57BL/6NCrlCrlj] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | mouse Ciz1 genomic DNA, Phage P1 loxP, yeast FRT (flipase recombination target) site |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |