Strain Data Sheet

RBRC05244

Strain Information

Image
BRC No.RBRC05244
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(Pitx2c-LacZ)24Hmd
Former Common nameB6-Pitx2c-lacZ Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hidetaka Shiratori and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2005. lacZ gene under the Pitx3 promoter (BAC RP23-194G9) was injected into the pronuclei of B6C3F2 fertilized eggs.
Strain descriptionPitx2c lacZ transgenic mice. These mice express a lacZ under the control of the Pitx2 promoter. Details unknown.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Biol., 395(2):331-41 (2014). 25224222

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UNmouse Pitx2c promoter

Ordering Information

Donor DNAE. coli LacZ gene, Mouse Pitx2 genomic DNA (BAC clone RP23-194G9), Phage P1 loxP site from BAC vector
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 395(2):331-41 (2014).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data