Strain Information | |
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Image | |
BRC No. | RBRC05280 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | nrd1 (Acc. No.CDB0466K) |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Eiichirou Nishi, Graduate School of Medicine, Kyoto University. |
Strain description | Nrd1, nardilysin, N-arginine dibasic convertase, NRD convertase 1 knockout mice (Nrd1 (lacZ)). Homozygous mutant mice mostly die within 48 hours after birth. Surviving mice show small brain and thin cerebral cortex, cortical thinning, hypomyelination, reduced grip strength, impaired coordination and impaired spatial working memory. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6N] |
References | Nat. Neurosci., 12, 1506-1513 (2009). 19935654 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Nrd1nardilysin, N-arginine dibasic convertase, NRD convertase 14Nrd1<tm1Eiin>targeted mutation 1, Eiichiro Nishi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin; |
Ordering Information | |
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Donor DNA | E. coli neo, yeast FRT (flipase recombination target) site, phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, E. coli lacZ, Rabbit beta globin pA signal, mouse nrd1 genomic DNA |
Research application | |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Eiichirou Nishi, E-mail: nishi@kuhp.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0466K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat. Neurosci., 2009 12(12):1506-13.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |