Strain Information | |
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Image | |
BRC No. | RBRC05284 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Prom1<tm1Toko>/Rbrc |
Former Common name | Prominin1(floxed-lacZ-DTA), Prominin1 (Acc. No. CDB0623K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Toru Kondo, RIKEN Center for Developmental Biology. |
Strain description | Prom1, prominin 1 knockout mice (Prominin1 (floxed-lacZ-DTA)). Exon 2, which contains the first ATG codon on the Prom1 locus was replaced with floxed-lacZ and DTA. Homozygous mice are viable and fertile. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6N] |
References | PLoS One, 4, e6869 (2009). 19718438 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DTdiphtheria toxin A (DT-A)5DT Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA5HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP |
Ordering Information | |
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Donor DNA | phage P1 LoxP, E. coli lacZ, rabbit beta globin pA, diphtheria toxin DT-A, pA, yeast FRT (flipase recombination target) site, mouse Prom1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Toru Kondo, RIKEN, E-mail: tkondo@igm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0623K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (PLoS One. 2009 4(8):e6869.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |