Strain Data Sheet

RBRC05284

Strain Information

Image
BRC No.RBRC05284
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prom1<tm1Toko>/Rbrc
Former Common nameProminin1(floxed-lacZ-DTA), Prominin1 (Acc. No. CDB0623K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toru Kondo, RIKEN Center for Developmental Biology.
Strain descriptionProm1, prominin 1 knockout mice (Prominin1 (floxed-lacZ-DTA)). Exon 2, which contains the first ATG codon on the Prom1 locus was replaced with floxed-lacZ and DTA. Homozygous mice are viable and fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesPLoS One, 4, e6869 (2009). 19718438

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prom1prominin 15Prom1 <tm1Toko>targeted mutation 1, Toru Kondo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DTdiphtheria toxin A (DT-A)5DT

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA5HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Ordering Information

Donor DNAphage P1 LoxP, E. coli lacZ, rabbit beta globin pA, diphtheria toxin DT-A, pA, yeast FRT (flipase recombination target) site, mouse Prom1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Toru Kondo, RIKEN, E-mail: tkondo@igm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0623K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (PLoS One. 2009 4(8):e6869.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data