Strain Information | |
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Image | |
BRC No. | RBRC05383 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(Prdm1-Venus)#Sait/Rbrc |
Former Common name | Brimp1-mVenus transgenic mouse, Blimp1-mVenus (Acc. No. CDB0460T) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6. |
Strain description | The transgenic mice having a Prdm1-mVenus containing a Venus reporter sequence with an Igk leader sequence, platelet derived growth factor transmembrane sequence and bovine growth hormone polyA signal that inserted in-frame after the initial ATG exon 3 of Pdrm1 BAC gene. Tg homozygous mice are viable and fertile. |
Colony maintenance | Carrier x Nocarrier [C57BL/6N] |
References | Reproduction, 136, 503-514 (2008). 18583473 Nature. 501(7466):222-226 (2013). 23913270 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)2GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence2HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mVenusYellow Fluorescent Protein (Aequorea victoria)2mVenusmouse Blimp1 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter myc-tagmyc-epitope tag2myc-tag Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mouse Igk leader sequence2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PDGF receptor anchoring domain2 |
Ordering Information | |
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Donor DNA | mouse Blimp1 genomic DNA, mouse Igk leader sequence, influenza virus hemagglutinin (HA) tag, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus) cDNA, c-Myc epitope tag, mouse PDGF receptor anchoring domain cDNA, Stop, Bovine growth hormone (BGH) polyadenylation signal |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0460T and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Reproduction. 2008 136(4):503-14.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. (5) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Aug 2013 |
BRC mice in Publications |
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No Data |