Strain Data Sheet

RBRC05387

Strain Information

Image
BRC No.RBRC05387
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(NDE-hsp-Pkd2*D509V/Venus)1Hmd
Former Common nameB6-NDE-Pkd2m2-venus Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Satoko Yoshiba and Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The transgene was microinjected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionThis transgenic mouse expresses the D509V variant Pkd2 and venus fusion gene on both sides of the node, node under the control of the mouse node-specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesScience, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkd2polycystic kidney disease 2UNPkd2mouse Nodal node-notochord enhancer, mouse Hsp68 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenus

Ordering Information

Donor DNAMouse Pkd2 gene cDNA, SV40 polyA signal, Aequorea victoria Venus (改良型GFP), Mouse Nodal node-notochord enhancer, Mouse Hsp68 promoter
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data