Strain Information | |
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Image | |
BRC No. | RBRC05388 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6C3-Tg(Wnt3-lacZ)5Hmd |
Former Common name | B6C3-Wnt3-lacZ Tg#5 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The transgene was microinjected into the pronuclei of B6C3F1 fertilized eggs. |
Strain description | An lacZ reporter gene was inserted into Wnt3 BAC clone at the initiating ATG codon. Expression of the transgene matches that of the endogenous Wnt3 at 8-cell mouse embryos. |
Colony maintenance | Carrier x Noncarrier [or Crossing to B6C3F1] |
References | Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319. 23271656 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UNmouse Wnt3 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA additional signalUN |
Ordering Information | |
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Donor DNA | E.coli LacZ, SV40 Poly A additional signal, mouse Wnt3 promoter |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319. |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |