Strain Data Sheet

RBRC05424

Strain Information

Image
BRC No.RBRC05424
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Drd1<tm2(IL2RA/mVenus)Koba
Former Common nameB6-Drd1/IL2RAmVenus KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine in 2010. A human IL2RA and mVenus fusion gene was transferred into 129 derived ES cells. The mutant mice were crossed to C57BL/6J for several times.
Strain descriptionThe human IL2RA and mVenus fusion gene was inserted into the start codon of Drd1 gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Neurosci., 31, 17169-17179 (2011). 22114284

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Drd1dopamine receptor D19Drd1<tm2(IL2RA/mVenus)Koba>targeted mutation 2, Kazuto Kobayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IL2RAhuman Interleukin-2 receptor alpha-subunit9IL2RA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mVenusYellow Fluorescent Protein (Aequorea victoria)9mVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal9

Ordering Information

Donor DNAMouse Dopamine D1 receptor (Drd1), SV40 PolyA signal, Human Interleukin-2 receptor alpha-subunit, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus)
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data