Strain Information | |
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Image | |
BRC No. | RBRC05432 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-D1Pas1<tm1(KOMP)Vlcg>/D4 |
Former Common name | B6-D1Pas1 KO#D4 |
H-2 Haplotype | |
ES Cell line | VGB6 [C57BL/6NTac] |
Background strain | C57BL/6NTac |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1 |
Ordering Information | |
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Donor DNA | Velocigene cassette ZEN-Ub1(E. coli lacZ, SV40 polyA, Phage P1 loxP sites, human ubiquitin C promoter, synthetic bacterial EM7 promoter (T7 phage), E. coli neo, mouse Pgk1 polyA), mouse D1Pas1 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Inoue H, Ogonuki N, Hirose M, Hatanaka Y, Matoba S, Chuma S, Kobayashi K, Wakana S, Noguchi J, Inoue K, Tanemura K, Ogura A. Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells. Biochem Biophys Res Commun 478(2) 592-8(2016) 27473657 Ogonuki N, Kyogoku H, Hino T, Osawa Y, Fujiwara Y, Inoue K, Kunieda T, Mizuno S, Tateno H, Sugiyama F, Kitajima TS, Ogura A. Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes. EMBO Rep 23(7) e54992(2022) 35587095 |