Strain Data Sheet

RBRC05440

Strain Information

Image
BRC No.RBRC05440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Muc21<tm2(Venus)Tats>/2
Former Common nameC57BL/6J-Muc21<tm2(Venus)Tats>/2TatsRbrc
H-2 Haplotype
ES Cell lineB6J-S1<UTR> [C57BL/6J]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tatsuro Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2011. C57BL/6J background. Main line (RBRC05439), Subline (RBRC05440).
Strain descriptionMucin 21/epiglycanin venus knock-in mice. A PGK-neo cassette and venus gene was inserted into Muc21 gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJJcl]
ReferencesGlycobiology, 18, 74-83 (2008). 17977904
J. Biol. Chem., 285, 21233-21240 (2010). 20388707

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Muc21mucin 2117Muc21<tm2(Venus)Tats>targeted mutation 2, Tatsuro Irimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)17Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;

Ordering Information

Donor DNAMouse Muc21, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, PhageP1 loxP, Yeast FRT, Aequorea victoria Venus
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorTatsuro Irimura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data