Strain Data Sheet

RBRC05442

Strain Information

Image
BRC No.RBRC05442
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ddhd2<tm1.1Katn>
Former Common nameB6-Ddhd2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuko Tani, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences in 2008. Targeted construct was electroporated into E14 ES cells. Exons 8 and 9 of Ddhd2 gene were removed by crossing with CAG-Cre (B6) mice. C57BL/6 and 129 mixed background.
Strain descriptionDdhd2, DDHD domain containing 2 (KIAA0725p) gene knockout mice. Exons 8 and 9 including a lipase-specific consensus sequence were deleted. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ddhd2DDHD domain containing 28Ddhd2<tm1.1Katn>targeted mutation 1.1, Katsuko Tani

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Ordering Information

Donor DNAP1ファージ loxP
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning of the BIOLOGICAL RESOURCE is published, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKatsuko Tani (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data